Vectors expressing chimeric Japanese encephalitis dengue 2 viruses
نویسندگان
چکیده
منابع مشابه
Evaluation of chimeric Japanese encephalitis and dengue viruses for use in diagnostic plaque reduction neutralization tests.
The plaque reduction neutralization test (PRNT) is a specific serological test used to identify and confirm arbovirus infection in diagnostic laboratories and monitor immunological protection in vaccine recipients. Wild-type (wt) viruses used in the PRNT may be difficult to grow and plaque titrate, such as the dengue viruses (DENV), and/or may require biosafety level 3 (BSL3) containment, such ...
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A molecular clone of Japanese encephalitis virus (JE virus) was derived from the JE virus Nakayama strain and used to produce infectious JE virus in cell culture. The engineered JE virus resembled the parental JE virus in cell-culture properties and was related closely to other JE virus strains based on nucleotide sequence analysis. The JE virus clone was used as a genetic background for constr...
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Two new chimeric flaviviruses were constructed from full-length cDNAs that contained tick-borne encephalitis virus (TBEV) CME or ME structural protein genes and the remaining genes derived from dengue type 4 virus (DEN4). Studies involving mice inoculated intracerebrally with the ME chimeric virus indicated that it retained the neurovirulence of its TBEV parent from which its pre-M and E genes ...
متن کاملCross-protection induced by Japanese encephalitis vaccines against different genotypes of Dengue viruses in mice
Dengue viruses (DENVs) and Japanese encephalitis virus (JEV) are closely related mosquito-borne flaviviruses that cause very high global disease burdens. Although cross-reactivity and cross-protection within flaviviruses have been demonstrated, the effect of JEV vaccination on susceptibility to DENV infection has not been well elucidated. In this study, we found that vaccination with the JEV in...
متن کاملJapanese encephalitis vectors in Thanjavur district, Tamil Nadu, India
Japanese encephalitis virus (JEV) is a mosquito-borne zoonotic pathogen. JEV is transmitted by paddy field breeding mosquitoes of the Culex vishnui subgroup, primarily by Culex tritaeniorhynchus vectors and amplified by pigs / Ardeidae birds in nature. Mosquitoes were sampled from identified villages during different season intervals from 2011 to 2014. Adult mosquitoes were collected which were...
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ژورنال
عنوان ژورنال: Acta virologica
سال: 2014
ISSN: 1336-2305
DOI: 10.4149/av_2014_04_340